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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
8-BROMO-1,3,4,5-TETRAHYDROBENZO[B]AZEPINE-2-ONE
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
885953-12-8
- 规格:
50mg
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文献和实验0.5g溶于10ml 100%的DMF内,混匀。4℃或分装存于-20℃,用前恢复至室温。 C.显色液:取A液40ul,加入到盛有10ml的0.1mol/l Tris-HCl(pH9.5,钤0.1mol/L NaCl、5mmol/L MgCl2)管内,充分混匀;再加入B液40ul,轻轻混合即可。最好用前新鲜配制。 NBT即四唑氮蓝(Nitro-Blue-Tetrazolium),MW=818,为深蓝色无定形微溶物质。BCIP即5-溴-4-氯-3-吲哚-磷酸盐(5-bromo-4-chloro
In Situ Hybridization Protocols
ug/ml of probe and continue incubation for a further 12-16 hours. N.B. We have found that the most effective way to carry out the hybridisation is in slide mailers. It is a good idea to thoroughly seal the lids of the slide mailers with parafilm
Anderson Lab In Situ Hybridization Protocols
Prehybridise for 3-4 hours at 60deg.C. Replace with 1-2ug/ml of probe and continue incubation for a further 12-16 hours. N.B. We have found that the most effective way to carry out the hybridisation is in slide mailers. It is a good idea to thoroughly
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