- ￥2600
- 源叶
- 中国
- T29540-1ml
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃（运输条件：冰袋低温运输）
- 英文名：
2-Naphthalenecarboxamid
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
192384-87-5
- 规格：
1ml
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文献和实验Troubleshooting for PCR and multiplex PCR
some/all of the above 3. Reaction was working before, but now I can't get any product. Make sure all PCR ingredients are taken in the reaction (buffer, template, Taq, etc) Change the dNTP solution (very sensitive to cycles of thawing and freezing, especially in multiplex PCR)
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
The specific complementary ass℃iation due to hydrogen bonding of single-stranded nucleic acids is referred to as "annealing": two complementary sequences will form hydrogen bonds between their complementary bases (G to C, and A to T or U) and form
. Estimation of the melting and annealing temperatures of primer: If the primer is shorter than 25 nucleotides, the approx. melting temperature (Tm) is calculated using the following formula: Tm= 4 (G + C) + 2 (A + T) G, C, A, T - number of respective
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