CCCI-01;215778-97-5;10mM in DM

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution

Stripping Western Blots

. 4mls/10cm plate), add fresh trypsin/ EDTA (e.g. 1ml/10cm plate), and incubate at 37oC as appropriate to loosen cells (e.g. 1-2min for NIH3T3, 2-5min for MDCK). 3. Add 4 volumes of soybean trypsin inhibitor (1mg/ml in serum-free medium) e.g. 4mls

阿司匹林对人γδT细胞杀伤消化系统肿瘤细胞的影响

h后加入阿司匹林(终浓度分别为0.1mmol/L、0.2mmol/L、0.4mmol/L、0.8mmol/L、1.6mmol/L、3.2mmol/L、6.4mmol/L、12.8mmol/L和25.6mmol/L)；设不加药物及无细胞的空白对照孔。继续孵育24h后每孔加人MTT液20μl，37℃孵育4h后弃上清，每孔加入DMSO 150μL，轻轻震荡10min使甲瓒充分溶解，在540nm波长酶标仪上测定各孔光吸收值(OD值)，求其平均值；同时计算细胞抑制率。1.2.3 γδT细胞和消化