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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
6'-Galactosyllactose
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
32581-31-0
- 规格:
2mg
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文献和实验. Methods 1, 27-29. 5. Houseman, B. T., Huh, J. H., Kron, S. J., and Mrksich, M . (2002) Peptide chips for the quantitative evaluation of protein kinase activity. Nat. Biotechnol. 20 , 270-274. 6. Lesaicherre, M . L., Uttamchandani, M., Chen, G
(optional; see Steps 21 and 37) Horizontal gel electrophoresis tank for agarose gels (for duplex PCR only; see Steps 40-53) Standard DNA sequencing gel apparatus with 31 x 38.5-cm glass plates, 0.4-mm spacers, and a shark's-tooth comb (for hot-stop PCR
PCR amplification of variable regions (SOE-Protocol)
by special gel filtration, using S-400 sephacryl columns from PHARMACIA (Cat. No. 27-5140-01). These columns take out primers and other PCR material. It is much faster than gel purification. Besides, gel purification can give bad ligation efficiency. Combine
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