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- 2025年10月29日
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文献和实验mRNA Amplification with T7 RNA Polymerase
Materials MessageAmp II aRNA Amplication Kit (Cat #1751, Ambion) 100% Ethanol RNA samples (e.g. 1 µg RNA per sample) Procedure Reverse transcription Verify that EtOH (24 mL) has been added to the Wash Buffer. Turn
T7 RNA Polymerase-Mediated Incorporation of 8-N3AMP Into RNA for Studying Protein-RNA Interactions
of a variety of proteins, its inability to serve as an efficient substrate for bacteriophage RNA polymerases apparently restricted its actual potential as a photocrosslinking agent. In this chapter, in vitro transcription conditions that allow for template
Synthesis of RNA by In Vitro Transcription
of a template that includes a bacteriophage promoter sequence (e.g. from the T7 coliphage) upstream of the sequence of interest followed by transcription using the corresponding RNA polymerase. In vitro transcripts are used in analytical techniques (e.g
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