E. coli DNA Ligase;R32236-200U

Transformation of E. coli by Electroporation

  Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate

Preparation of Chromosomal DNA from E. coli

This chapter describes a simple and rapid way of extracting and purifying chromosomal DNA from E. coli and many other species of bacteria. This procedure is essentially a simplified version of that described by Marmur in 1961 (1 ). The cells

Maxiprep of plasmid DNA from E. coli

Grow a single colony of E. coli overnight in 50mL LB broth + selective markers at 37°C. The next morning, put 850μL of the culture in each of two Eppendorf tubes, add 150μL sterile 50% glycerol, and store at -80°C. Pour as much culture as will fit