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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
19,20-epoxy-16,19-kauranediol
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
260999-77-7
- 规格:
5mg
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文献和实验Crystallization of Kinesin Family Motor Proteins
)Vapor diffusion15 mg/ml protein co-crystalized with 5 mM AMPPCP + 20 mM MgCl2 in reservoir buffer (RB3) composed of 30% w/v PEG4000, 100 mM Tris-HCl pH 8.5, 200 mM sodium acetateKikkawa et al., 2001Nkin 1-355Sitting Drop 19°C7.5-15 mg/ml protein in 20 mM
tubes, remove air bubbles, seal with rubber stoppers, and crimp properly. 11a. Centrifuge at 60,000 rpm to 16-20 hours at 15-20degC in the Sorvall OTD-75B ultracentrifuge (DuPont) using the T-865 rotor. 12a. Visualize the ethidium bromide
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
for one reaction: The primary PCR cycling conditions are as follows: (a) 95°C for 1 min 30 s (b) 10 cycles of ●● 95°C for 5 s ●● 70°C for 3 min 10 s with decreasing 0.5°C each cycle(c) 20 more cycles of ●● 95°C for 5 s ●● 65°C
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