维生素B12;68-19-9;100μg/ml in met

Stripping Western Blots

- 1 μg/ml (Note: Molecular Probes is odd in that they sell you a number of ‘units’, rather than a μg amount, of product. We dissolve 1 vial (300U) in 1.5ml methanol, and use a 1:100 dilution of this. Most other companies sell a μg

支原体污染的特点及检验（1）

过滤膜过滤灭菌。勿用高压蒸汽灭菌。　　· Hoechst 33258 stock solution（100X）︰称取5.0 mg bisbenzimide和10.0 mg thimersol溶于100 ml无菌HBSS，置于棕色瓶中，室温下以电磁搅拌器搅拌30分钟至完全溶解后，以1ml分装至1.5ml小离心管中，贮存于-20℃。　　· Hoechst 33258 working reagent︰取1 ml Hoechst 33258 stock solution，加入sterile 100 ml HBSS中

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit