丁-3-炔-2-胺盐酸盐;42105-26-0;95%;Y9

【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离

-stranded DNA linker,namely splinkerette, to the digested DNA. The insertion flankingsequences are then PCR amplified using a pair of primers specific tothe integrated DNA and the splinkerette, respectively (26). Splinkerette-PCR features a hairpin structure

v468 Chapter 2 Wnt活性蛋白分离及应用

Filtration 1. Sample: 5–10 mL pooled Wnt3A-containing fractions fromSection 2.3.2 . 2. HiLoad 26/60 Superdex 200 preparative grade. 3. Buffer: 1× PBS, 1% (w/v) CHAPS. 4. Syringe (30 mL) with 0.2-μm pore size filter. 2.3.4. Step 4: Heparin

HIGH RESOLUTION GENETIC FOOTPRINTING

volume (final concentration, amount of stock added to make 250 ml solution) 50 mM Tris pH 8.0, 12.5 ml of 1 M stock 10 mM EDTA, 5 ml of 0.5 M stock 140 mM NaCl, 7 ml of 5 M stock 10% glycerol, 31.25 ml of 80% stock 10 mM DTT, 385 mg 1 mM PMSF, 4.35 ml