异丙醇锂;2388-10-5;1.0M in hexanes

酵母双杂交的实验步骤【分享】

pLexA-X(0.1 lmg) 3-5mB. pB42AD-Y(0.1 C. 10mg/ml Sperm lmDNA*(0.1mg) 10 * 新配制时水浴煮沸20min后，插入冰浴，保存于-20℃。 l用1×TE/LiAc重悬的酵母感受态细胞，振荡混匀。m7. 每管加入100 lm8. 各加入600 PEG/LiAc，振荡混匀，30℃恒温，250rpm振荡培养30min。 lm9. 各加入70 DMSO缓和倒置混匀。42℃热休克

AFLP: not only for fingerprinting, but for positional cloning

+ (no BSA) 1.0 µL (5-10 U/µL) T4 polynucleotide kinase (PNK) 24.0 µL dH2O §50 µL labeling rxn makes enough "hot" primer for 100 PCR rxns. † The basic EcoRI-primer is: 5'-AGA CTG CGT ACC AAT TCx yz-3' where, x, y, and z represent

AFLP: not only for fingerprinting, but for positional cloning

.   Extraction Buffer 50 mM Tris, pH 8.0 10 mM EDTA, pH 8.0 100 mM NaCl 1.0 % (w/v) SDS 10 mM ß-mercaptoethanol (add just before use) 3) Add 150 µL 5 M K-acetate, vortex, incubate on ice for 20 min. Spin @ 12K x g