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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 英文名:
Chrysene solution
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
218-01-9
- 规格:
2ml
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文献和实验than formaldehyde-fixed gonads. Fix dissected gonads in 3 ml of cold (-20¡) methanol for 5 min. Using a pasteur pipette, transfer to a glass conical glass tube, add 2ml PBTw and spin 1 min in a clinical benchtop centrifuge at setting 2. Remove the supernatant
96-well RNA In Situ Hybridization Protocol
Noordermeer and Casey KopczynskiI.PREPARATION OF EMBRYOS1. Embryos were collected for 24 hours and washed 3X with 0.02% Triton X (Tx)(15ml for up to 2ml embryos in a 50ml falcon tube).2. Dechorionate 5 min. in 50% bleach.3. Wash 3X with 0.02% Tx.4. Fill tube
In Situ Hybridization Protocols
: For 100ml 50% Formamide 50ml 5x SSC 25ml 20xSSC 1mg/ml Yeast tRNA 2ml of 50mg/ml in DEPC-H2O 100ug/ml Heparin 10mg 1xDenhardt's Solution 1ml 100x
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