WAY-643116;850020-01-8;10mM in

重组DNA的分离、克隆与测序实验手册

9.0 4 ml 1 M (NH4)2SO4, pH 9.0 25 mM MgCl2, pH 7.0 1 ml 1 M MgCl2, pH 7.0 5% DMSO 2 ml DMSO 17 ml ddH2O 40 ml The 5X Taq reaction buffer will be added separately with the A, C, G, and T nucleotide mixes

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution

Cajal Body Isolation Protocol

sucrose, 0.5 mM MgCl2 S3 solution: 0.5M sucrose, 25 mM Tris-HCl pH 9.0 SP1 buffer: 1M sucrose, 34.2% Percoll, 22.2 mM Tris-HCl pH 7.4, 1.11 mM MgCl2 SP2 buffer: 20% Percoll, 10mM Tris-HCl pH 7.4, 1% Triton X100, 0.5 mg/ml heparin HT buffer: 10mM