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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
CL-417513
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
313986-65-1
- 规格:
1ml
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文献和实验buffer; 1ml of ChIP high salt buffer; 1ml of ChIP wash buffer; (note 4)Pulldown (2), Flag-tag specific After the last wash elute the complex by adding 100µl of ChIP elution buffer. Incubate the samples at 65°C for 10 minutes
CTABTECHNIQUE Method Schedule Protocol(JPB)FOR DNA
8.0 100ml Tris-Cl stock (1M) 1.4M NaCl 280ml NaCl stock (5M) make up to 1 Litre with water, pH 7.5 - 8.0, and autoclave 0.2% Mercaptoethanol Wash Buffer 76% Ethanol 10mM NH4 Ac DNA Extraction
CTAB TECHNIQUE / Method / Sched
, pH 7.5 - 8.0, and autoclave+ 0.2% Mercaptoethanol Wash Buffer 76% Ethanol 10mM NH4 Ac DNA Extraction 1. Preheat 5ml CTAB (add 10µl mercaptoethanol to each 5ml CTAB) in a blue-topped 50ml centrifuge tube at 60-65℃. Remove and discard midribs
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