BMS-309403 sodium;2802523-05-1

Filter Binding Assay for EBNA-1

staining by UV.Quantify percent of each band present by comparison to input DNA fragment used.(Optional) Quantify protein in each fraction by SDS-PAGE/Western.Binding Buffer : 10mM HEPES 10mM MgCl2 150mM NaCl Add 5% DMSO fresh Wash

ELISA protocol

Reagents : PBS (pH 7.3) Coating Solution: 0.1 M Sodium Carbonate (pH9.6) with 0.02% Sodium Azide Wash Solution: 0.9% NaCl-0.05% Tween 20 (NaCl/T) PBS-TA: PBS-Tween 20 (0.05%) - sodium azide (0.02%) pH 7.3 AP

Yeast Cell Lysates

from stocks to give 50 fold dilution. Example: 20 ul of stock per 1ml buffer. Use IMMEDIATELY after adding protease inhibitors.   SUMEB BUFFER (1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA, 0.01% bromophenol blue) TO MAKE 100ml: 1.0ml 1M