BA-53038B;2306195-65-1;10mM in

人APC和TSC－2 cDNA的长5＇RACE

 图2 APC 5＇末端的扩增。用对应APC mRNA的位置5846的GSP反转录1mg Hela总RNA得到APC mRNA。cDNA被纯化、加尾，和用对应位置1797（A带）和位置1632（B带）的引物和AAP一起，用ELONGASE酶混合物来扩增（94℃ 30s，接着35个循环的94℃ 15 s，55℃ 30 s，68℃ 5 min）。初步PCR用TE缓冲液稀释100倍，取1ml用引物APC－1632（A带）或引物APC－1242（B带）和AUAP分别重新扩增（30个循环），每个PCR

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

buffer; 1ml of ChIP high salt buffer; 1ml of ChIP wash buffer; (note 4)Pulldown (2), Flag-tag specific After the last wash elute the complex by adding 100µl of ChIP elution buffer. Incubate the samples at 65°C for 10 minutes

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution