AGN 194310;229961-45-9;10mM in

人APC和TSC－2 cDNA的长5＇RACE

DMSO（终浓度为0.2％）不影响PCR。 图4 TSC－2 cDNA的长5＇RACE。初步PCR产物（带1和带3）和巢式PCR产物（带2和带4）的琼脂糖凝胶分析。用5µg Hela总RNA，引物TSC－2－5472，45℃完成cDNA合成，dC加尾反应用了10µl GLASSMAX纯化的cDNA，并包含10％的DMSO，用1µl 加尾反应产物直接PCR扩增[94℃

Filter Binding Assay for EBNA-1

Wash one 0.45um Millipore nitrocellulose filter (Cat# HAWP01300) in 1ml 0.4M KOH for 1 min; wash in 3ml dH2 O for >1min. Assemble filter apparatus: white plastic bottom support/funnel, nitrocellulose filter, white plastic top with syringe adapter

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

H20 ChIP elution buffer       20ml final volume:       500µl 2M Tris       2ml 10% SDS       400µl 0.5M EDTA       17.1ml H2O cold lysis buffer       1mM PMSF       20units RNasin       10mM Tris-HCl pH 7.4       10mM NaCl       3mM MgCl2