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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
AS2863619 free base
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
2241300-50-3
- 规格:
1ml
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文献和实验PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
sample DNA templates were amplified at different annealing temperatures as shown: note that while plasmid is amplified from 37 to 55℃, HPV DNA is only specifically amplified at 50℃.Primer Length The optimum length of a primer depends upon its (A+T
In Situ HYBRIDIZATION TO TISSUE SECTIONS
7.5 127g Trizma HCl 47.2g Trizma Base H2O to 1L 1M Tris-HCl, pH 8.0 177.6g Trizma HCl 106g Tizma Base H2O to 1L Triton X-100 (SIGMA X-100) WDTT 1X Salts 50% Formamide 10mM DTT Xylene
the gel: dissolve 0.8g agarose in 100mL of 10mM NaPO4 pH 7.0 (5mL of 0.2M NaP04 pH 7.0 + 95mL "DEPC" H2 O). Make sure gel box and comb are RNase-free by treatment with 3% H2 O2 or commericial RNase removal product (e.g. RNase Zap from Ambion).
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