Fanotaprim;2120282-75-7;10mM i

Northern Blot: Glyoxal/DMSO

1.25uL 1.5uL DMSO 7.5uL 10uL 12.5uL 15uL 6M(40%) Glyoxal* 3uL 3.75uL 2.25uL

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

H20 ChIP elution buffer       20ml final volume:       500µl 2M Tris       2ml 10% SDS       400µl 0.5M EDTA       17.1ml H2O cold lysis buffer       1mM PMSF       20units RNasin       10mM Tris-HCl pH 7.4       10mM NaCl       3mM MgCl2      

In Situ HYBRIDIZATION TO TISSUE SECTIONS

1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine