EMT inhibitor-1;1638526-21-2;1

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

and centrifuge 4 minutes at 2000 RPM;Remove the PBS+1/1000 PMSF by aspiration and re-suspend cell pellet in 1ml ChIP lysis buffer plus the protease inhibitors 1/1000 PMSF and 50 units of RNAse inhibitor. The final volume of cell lysis buffer should be sufficient

Eccles:Protein Lysates from Cells in Culture

Count cells Spin cells in 1mL PBS at 4 degrees C, 8000g for 10mins Discard supernatant and resuspend cells in cell lysis buffer containing 1x Complete protease inhibitor. I used to do 1x105 cells/uL but can do whatever you want

v468 chapter 5 糖原合成酶激酶-3 的抑制

of the serine/threonine kinaseAkt, which will phosphorylate GSK-3 at specific serine residues(Ser21 in GSK-3a and Ser9 in GSK-3b), leading to the inactivationof GSK-3 kinase activity (10) . However, this phosphorylationand inactivation of GSK-3 by Akt