- ￥4510
- 源叶
- 中国
- T96304-1ml
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃（运输条件：冰袋低温运输）
- 英文名：
EMT inhibitor-1
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
1638526-21-2
- 规格：
1ml
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文献和实验and centrifuge 4 minutes at 2000 RPM;Remove the PBS+1/1000 PMSF by aspiration and re-suspend cell pellet in 1ml ChIP lysis buffer plus the protease inhibitors 1/1000 PMSF and 50 units of RNAse inhibitor. The final volume of cell lysis buffer should be sufficient
Eccles:Protein Lysates from Cells in Culture
Count cells Spin cells in 1mL PBS at 4 degrees C, 8000g for 10mins Discard supernatant and resuspend cells in cell lysis buffer containing 1x Complete protease inhibitor. I used to do 1x105 cells/uL but can do whatever you want
of the serine/threonine kinaseAkt, which will phosphorylate GSK-3 at specific serine residues(Ser21 in GSK-3a and Ser9 in GSK-3b), leading to the inactivationof GSK-3 kinase activity (10) . However, this phosphorylationand inactivation of GSK-3 by Akt
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