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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃,干燥(运输条件:冰袋低温运输)
- 英文名:
GDC-0575 dihydrochloride
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
1657014-42-0
- 规格:
1ml
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文献和实验Efficient Transformation of Yeast - LiAc Method
efficiency using the following protocol: 10xLiAc: 1M LiAc pH 7.5 (adjust with HAc) 10xTE: 0.1M Tris-Cl (pH 7.5), 10mM EDTA 50% PEG 50% PEG 4000 in water (autocalve ONCE only) PEG/LiAc sol. 8ml 50% PEG, 1ml 10
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution
后, 用1/3体积的冰冷TB溶液悬浮, 在冷10分钟再次离心回收菌体用1/12.5体积的TB悬浮, 添加最终浓度为7%的DMSO, 再冷却10分钟0.1-1毫升分装, 直接在液氮中冻上.在液氮或-80度保存. TB溶液*TRANSFORMATION BUFFER PIPES 3.0G 10mM CaCl2.2H2O 2.2g 15mM KCl 18.6g 250mM add water up to 950ml 用 5N KOH
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