GPR120 Agonist 3;1599477-75-4;

Purification of recombinant sBRF M166L

, prepare the Ni-NTA resin for BRF binding. Transfer 12 mls of Ni-NTA affinity bead slurry (50% beads; 6 ml of beads total) to a 50ml conical tube and centrifuge 3min at 3K RPM in the GPR centrifuge. Equilibrate the beads 2X with Lysis buffer

Purification of recombinant sBRF M166L

for BRF binding.Transfer 12 mls of Ni-NTA affinity bead slurry (50% beads; 6 ml of beads total)to a 50ml conical tube and centrifuge 3min at 3K RPM in the GPR centrifuge. Equilibrate the beads 2X with Lysis buffer by resuspension and centrifugation. Add

In Situ HYBRIDIZATION TO TISSUE SECTIONS

1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine