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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
Telomerase-IN-2
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
1610878-54-0
- 规格:
1ml
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文献和实验9.0 4 ml 1 M (NH4)2SO4, pH 9.0 25 mM MgCl2, pH 7.0 1 ml 1 M MgCl2, pH 7.0 5% DMSO 2 ml DMSO 17 ml ddH2O 40 ml The 5X Taq reaction buffer will be added separately with the A, C, G, and T nucleotide mixes
Alkaline Lysis and CsCl Purification of BAC DNA for Transgenic Production.
4. Wash cells by resuspending in 25 mls of ICE COLD 10mM NaCl (0.1ml 5M into 50ml final) per 250 ml bottle, pellet at 3800 rpm for 10minutes at 4°C. 5. Decant supernatant, (freezing the pellet at minus 80°C overnight at this step increases cell
Purification of recombinant sBRF M166L
of beads will not be very firm so it seems best to remove as much supernatant as possible with a pipette and don’t worry if not all the sup. can be removed). Save this supernatant in case there is a problem with the BRF binding. Resuspend the beads
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