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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
Antifungal agent 1
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
1265166-14-0
- 规格:
1ml
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文献和实验In Situ HYBRIDIZATION TO TISSUE SECTIONS
the nitrogen touch the O.C.T.). Put the chuck with embryos in the cryostat so they can come up to temperature. Cutting frozen sections Take the chuck (plus embryos!) out of the cryostat (they should be at cryostat tempera- ture, -14 to -19
? Answer, continued. I don't know how good a buffer sea water is, but it's unlikely to be as robust as 0.1M phosphate. In a fixative the osmolarity is more important than the pH, but for a slowly acting agent like formaldehyde or a slowly penetrating
Hybridisation to RNA in Tissue Sections
The DNA which will act as a template must be subcloned into a vector containing promoters for RNA polymerase, eg. Bluescript has T3 and T7 promoters. Ideally, the insert DNA should be cloned into Bluescript in both orientations so you can use the same RNA
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