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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
WAY-107407
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
1164469-67-3
- 规格:
1ml
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文献和实验In Situ HYBRIDIZATION TO TISSUE SECTIONS
1.0ml 10mM rGTP 1.0ml 0.5mM rUTP 1.0ml 0.75M DTT 1.0ml RNase-Block (25U/ml) 2.5ml 35S-rUTP (100mCi) 1.0ml T3 or T7 polymerase (10U) Vortex, spin down and incubate 1-2h @ 30 degrees C. Incorporation should be 70-90%. Determine
Hybridisation to RNA in Tissue Sections
polymerase to generate both the sense and anti-sense strands (and eliminate variability between the T7 and T3 RNA polymerases - one way to try to match sense against anti-sense). The template must be linearised when undertaking in vitro transcription
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