- ￥431
- 源叶
- 中国
- T94160-1ml
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃（运输条件：冰袋低温运输）
- 英文名：
Puromycin (CL13900) 2HCl
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
58-58-2
- 规格：
1ml
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文献和实验Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别
T℃OS-M6 cells grow in 6-well plates, 2ml media total T℃V1PD cells grow in 100mm plates. 1: Aspirate off the media from the cells and was the cell monolayers with 2x5ml (To 100mm dish) or 2x1ml (to 6-well plates) ice
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别提取细胞核和细胞质RNA
T℃OS-M6 cells grow in 6-well plates, 2ml media totalT℃V1PD cells grow in 100mm plates.1: Aspirate off the media from the cells and was the cell monolayers with 2x5ml (To 100mm dish) or 2x1ml (to 6-well plates) ice cold TD (4o C is OK
Fractionation of Nuclear and Cytoplasmic RNA by Trizol--分别提取细胞核和细胞质RN
concentration of phenol is 38% x 1.2 = 45.6%. Add water to 200 ml total volume. pH ~ 4.5. Wrap with aluminum foil. RNA precipitation solution: 200 ml NaCl 1.2 M 14.026 g FW 58.44 Sodium Citrate 0.8M 47.056 g FW 294.10 Autoclaved (can’t be filtered
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