CASIN;425399-05-9;10mM in DMSO

Yeast Cell Lysates

from stocks to give 50 fold dilution. Example: 20 ul of stock per 1ml buffer. Use IMMEDIATELY after adding protease inhibitors.   SUMEB BUFFER (1% SDS, 8M Urea, 10mM MOPS, pH 6.8, 10mM EDTA, 0.01% bromophenol blue) TO MAKE 100ml: 1.0ml 1M

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

H20 ChIP elution buffer       20ml final volume:       500µl 2M Tris       2ml 10% SDS       400µl 0.5M EDTA       17.1ml H2O cold lysis buffer       1mM PMSF       20units RNasin       10mM Tris-HCl pH 7.4       10mM NaCl       3mM MgCl2      

Eccles:Protein Lysates from Cells in Culture

  Cell Lysis Buffer 5mL 0.1M Tris HCl pH 8 (10mM) 0.44g NaCl (150mM) 0.02g EDTA (1mM) 0.5mL nonidet P40 (1% w/v) 0.05g SDS (0.1% w/v) Make up to 50mL with MQH2 O and filter sterilise. Store at 4 degrees C. This cell lysis