Retapamulin;224452-66-8;10mM i

【原创】制备高效感受态细菌方法

此方法是我在国外的一个实验室工作时所用的。简单、批量、转化效率高。 Simple and Efficient Method (SEM) to Make Competent Cells Preparation of Frozen Competent of DH5α 1) 250 ml TB solution 10mM Pipes or 10 mM Hepes, 0.65g 15 mM CaCl2, 0.55g 250 mM KCl, 4.66g *55 mM MnCl2

高效制备感受态细胞

如果用来建库或者转化比较珍贵的材料可以试一下这种感受态！效率非常高！ Simple and Efficient Method (SEM) to Make Competent Cells Preparation of Frozen Competent of DH5α （1） 250 ml TB solution 10mM Pipes or 10 mM Hepes, 0.65g 15 mM CaCl2, 0.55g 250 mM KCl, 4.66g *55

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution