非布索坦;144060-53-7;10mM in DMSO;

immunofluorescence staining

antibodies with no known specificity tagged withthe same fluorochromes as the test antibodies. Sometimes when dealing with suspensions where there will be negative and positive cells such as peripheral T-Cells, it is permissible to consider the unstained

流式细胞分析分选术

的上样条件。细胞量为1×106 个/ml，1ml。 2) 上机前，应对样本进行混合，以使样本均一(注意使用排枪吹打，勿产生气泡)。 3) 以上protocol 仅针对于悬浮细胞。 4) 药物刺激浓度需先做梯度检测。 第七章 流式细胞分析分选术 42 2. Cell Cycle Assay 常用的实验细胞株包括：293(T)，MCF7，U2OS，Saos，Hela 等 以U2OS 细胞为例 2.1 细胞培养及铺板 1) 细胞株及材料： a) 细胞

Stripping Western Blots

. 4mls/10cm plate), add fresh trypsin/ EDTA (e.g. 1ml/10cm plate), and incubate at 37oC as appropriate to loosen cells (e.g. 1-2min for NIH3T3, 2-5min for MDCK). 3. Add 4 volumes of soybean trypsin inhibitor (1mg/ml in serum-free medium) e.g. 4mls