马来酸氯苯那敏;113-92-8;10mM in DMSO;

Amplification and Labelling with Cy dyes

1.Two rounds of DNA synthesis to incorporate fixed sequences Prepare the following: Reaction tube containing the following: 7ml DNA to be amplified and labelled 2ml 5X T7 Sequenase Buffer 1ml 50mM Pimer 1 (GTTTCCCAGTCACGATCNNNNNNNNN) Reaction

v468 chapter 5 糖原合成酶激酶-3 的抑制

) solution in waterand freeze aliquots at –20°C. 5. Water-saturated isobutanol. Store at RT. 6. Running buffer (10×): 250 mM Tris, 1.92 mM glycine, and1% (w/v) SDS. Store at RT. 7. Prestained molecular weight markers: Rainbow marker (GEHealthcare

Stripping Western Blots

. 4mls/10cm plate), add fresh trypsin/ EDTA (e.g. 1ml/10cm plate), and incubate at 37oC as appropriate to loosen cells (e.g. 1-2min for NIH3T3, 2-5min for MDCK). 3. Add 4 volumes of soybean trypsin inhibitor (1mg/ml in serum-free medium) e.g. 4mls