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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 英文名:
3,4-DIAMINO-6-METHYL-1,2,4-TRIAZIN-5(4H)-ONE
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
52553-11-4
- 规格:
5mg
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文献和实验Single tube confirmation PCR protocol
containing 60 U/ml of Zymolyase (This solution is generated by resuspending 6 mg of 100T Zymolyase in 10 mls of water. Ordering information: Seikagaku Corp., #120493-1). - Sterile yellow tips work great for this task. - Repeat this step
DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
. Do one case at a time. If normal is being dissected do this first. Scrape away areas that you dont want away from the sample (use a #11 blade). Take a new #15 blade and dip (don't dunk) tip of blade into the labeled tube with pcr pk
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
Digestion, Addition, Ligation, PCR Amplification, and Examination) (see Notes 8–10) 1. Digestion: Digest 2 mg of genomic DNA with 7.5 U of Taq Iat 65°C for 4 h in a final volume of 50 mL, followed by another4-h digestion with 7.5 U BspLU11 I and 10 U
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