高铼酸 溶液;13768-11-1;75-80 wt. %

AFLP: not only for fingerprinting, but for positional cloning

of isopropanol. Immediately spin down nucleic acids by centrifugation @ 12K x g for 1 min. [Some small pieces of tissue may pellet at this stage, but they will be removed in the next step.] 5) Wash pellet w/ 75% EtOH and resuspend in 200 µL H2O. Spin @ 12K x g

AFLP: not only for fingerprinting, but for positional cloning

, but they will be removed in the next step.] 5) Wash pellet w/ 75% EtOH and resuspend in 200 µL H2O. Spin @ 12K x g , 2 min. Remove 180 µL of supernatant to new tube and add 20 µL 3 M Na-acetate (or K-acetate). Mix and add 500 µL EtOH, then incubate @ -20°C for _ 10

M13 噬菌体衣壳蛋白改造在改良噬菌体展示技术中的应用

.1.1)。 ( 9 ) PEG/NaCl ( 见 12.2.1.1)。 ( 10 ) SOC 培养基：5 g 细菌用酵母抽提物、20 g 细菌用胰蛋白胨和 0.5 g 氯化钠及 0.2 g 氯化钾；加水到大约1 L，用氢氧化钠调节 pH 到 7.0；高压灭菌；然后加入 5 ml 2.0 mol/L 高压灭菌过的氯化镁溶液及 20 ml 超滤灭菌过的 1.0 mol/L 葡萄糖溶液；并将最终体积调至 1 L。 ( 11 ) M13KO7 辅助噬菌体（见 12.2.1.1)。 2.1.4 制备电穿孔法感受