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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
WAY-332004
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
902586-30-5
- 规格:
1ml
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文献和实验H20 ChIP elution buffer 20ml final volume: 500µl 2M Tris 2ml 10% SDS 400µl 0.5M EDTA 17.1ml H2O cold lysis buffer 1mM PMSF 20units RNasin 10mM Tris-HCl pH 7.4 10mM NaCl 3mM MgCl2
Western Blotting with Biotinylated Antibodies
in all steps using HRPO (horseradish peroxidase) conjugates. 1.Decant the primary antibody solution, add wash buffer (10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20), and wash for 30 minutes with agitation, changing the wash buffer every 3-5 minutes. 2.Dilute
Western Blotting with Biotinylated Antibodies
minutes with agitation, changing the wash buffer every 3-5 minutes. Dilute the strepavidin:HRPO conjugate at the supplier’s recommended dilution in blocking buffer (3% BSA, 10mM Tris pH 7.5, 100mM NaCl, 0.1% Tween 20). Decant the wash buffer
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