WAY-332005;901722-67-6;10mM in

In Situ HYBRIDIZATION TO TISSUE SECTIONS

DNase (1U/ml) to the 24ml reaction mixture and incubate 30' @ 37 degrees C. Stop the reaction: +60ml 10mM EDTA, 10mM Tris-HCl (pH 7.4), 0.2% SDS +1.67ml 5M NaCl +1.0ml 1M DTT Incubate 10-15' on ice Hydrolyze the probe (ideal

Hybridisation to RNA in Tissue Sections

0.8 333 mM 10mM GTP 0.8 333 mM 50 mM UTP (cold) 0.67 1.67 mM hot UTP32 (100 mCi) 10 1.67 mM linearised template DNA