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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃(运输条件:冰袋低温运输)
- 英文名:
WAY-600632
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
438018-70-3
- 规格:
1ml
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
B5 vitamines (1ml of 1000x stock) (SIGMA; #G-2519) Gamborg''s vitamine powder, to prepare the 1000x stock disolve 11.2g in 100ml water. 5% sucrose adjust to pH 5.7 before autoclaving after autoclaving add: - Benzylamino Purine (BAP
In Situ HYBRIDIZATION TO TISSUE SECTIONS
, pH 8) +10 ml heptane The MeOH/EGTA/heptane mix should be prechilled to -70 degrees C on dry ice. It takes awhile to come down to temp. so place it on dry ice about 1 hr earlier. One way to transfer the embryos to the cold mix
M buffer - 1 hour. (Mix equal quantities of 2% aqueous OsO4 and 0.4M buffer and use immediately.) Rinse in 0.2M buffer - 5 mins. x 2. Dehydrate in 70% ethanol - 20 mins. x 2. (May be stored overnight at this stage if absolutely necessary
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