WAY-638741;796085-51-3;95%;S58

In Situ Hybridization Using Digoxigenin Labeled Probes

  In Situ Hybridization Using Digoxigenin Labeled Probes The Cytogenetics Core performs polytene chromosome in situ hybridizations to polytene chromosomes to serve the needs of the BDGP's various projects. The Core prepares probes

LINEAGE ANALYSIS USING RETROVIRAL VECTORS

and ballssterile Tissue retractorsTissue scissorsLactated Ringer's solution (LR) containing Penicillin/StreptomycinFiberoptic light sourceVirus stockAutomated microinjector1-5 ml micropipettes3-0 Dexon sutureTissue stapler1. Mix ketamine and xylazine 1:1 in a 1 ml

PCR

in a thermocycler. Heat sample to 95°C, then add 0.5 -1 ul of enzyme (Taq, Tli, Pfu etc.). Add a few drops of mineral oil.3.Start the PCR cycles according the following schemes:(1) denaturation - 94 ° C, 30-90 sec.(2) annealing - 55 °C (or -5° Tm), 0.5-2 min