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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 英文名:
WAY-622134
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
409356-56-5
- 规格:
5mg
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文献和实验DNA EXTRACTION FROM MICRODISSECTED PARAFFIN SECTIONS
) through the oil to sample. (It's 0.3 ul per 15ul original volume, so adjust as necessary). Incubate overnight at 55 C. Day 3: (Repeat:) Add 0.3 ul fresh conc Proteinase K (20 mg/ml stock) through the oil to sample. (It's 0.3 ul per 15 ul original
Single tube confirmation PCR protocol
. For example, add 5 µl of the Zymo solution from isolate #1 to PCR tubes 1-5, 5 µl of the Zymo solution from isolate #2 to PCR tubes 6-10 and so on. - Make a PCR master mix by combining: 638 µl water, 110 µl 10 x Taq Buffer, 11 µl NTP's, and 11 µl Taq
1.103.90±0.95(1.20-5.00)138±2.90134_2.60(128-145)5.25±0.135.40±0.15(4.85-5.85)108±0.60107±0.55(105-110)26.2±2.1024.8±2.30(20.2-31.5)5.60±1.616.55±1.30(2.30-9.20)5.60±0.407.40±0.50(3.20-8.50)3.11±0.371.38±0.28(0.80-3.90)大鼠0.35±0.020.24±0.07(0.00-0.55)28
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