- ￥279
- 源叶
- 中国
- S58022-1mg
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°C
- 英文名：
1-(4-Ethylpiperazin-1-yl)-2-(((5-methyl-2-phenyloxazol-4-yl)methyl)sulfinyl)ethan-1-one
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- CAS号：
957674-68-9
- 规格：
1mg
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文献和实验【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
for one reaction: The primary PCR cycling conditions are as follows: (a) 95°C for 1 min 30 s (b) 10 cycles of ●● 95°C for 5 s ●● 70°C for 3 min 10 s with decreasing 0.5°C each cycle(c) 20 more cycles of ●● 95°C for 5 s ●● 65°C
Northern Blotting (Howell Lab)
into (14x95 mm) polyallomer tubes with 4 ml CsCl buffer. Balance and top off tubes with more GITC buffer. Spin tubes on the SW40Ti rotor for 20 hr at 32,000 rpm (18℃). Pour off liquid and keep tubes inverted because the RNA pellets
Methods2.1. Material and reagentsDuolbecco’s phosphate buffered saline (PBS), Medium 199 with Earle’s salts and 25mM HEPES, collagenase Type II (from Clostridum histolyticum), antibiotic/antimyotic solutuion (containing 10,000U penicillin, 10,000µg
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