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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C
- 英文名:
N-(4-fluorophenyl)-2-{[5-(furan-2-yl)-4-phenyl-4H-1,2,4-triazol-3-yl]sulfanyl}acetamide
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
331710-55-5
- 规格:
10mg
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文献和实验Preparation of Mouse Tail DNA for Dot Blots or PCR
These procedures were originally devised in Richard Palmiter's lab for use with tail dots (DNA spotted onto a nitrocellulose filter and probed for a transgene; quicker than doing southerns), and subsequently modified for PCR preps. 1.Cut off tail
Preparation of Mouse Tail DNA for Dot Blots or PCR
Preparation of Mouse Tail DNA for Dot Blots or PCR These procedures were originally devised in Richard Palmiter's lab for use with tail dots (DNA spotted onto a nitrocellulose filter and probed for a transgene; quicker than doing
IN SITU HYBRIDIZATION ON FROZEN SECTIONS
Remove slides from freezer, thaw for 5 min. at 55°C. Fix 10 min. in 4% paraformaldehyde, 4°C. Wash 5 min. in 0.5x SSC, RT. Immerse slides in proteinase K solution , 1-5 µg/ml in RNase Buffer for 10 min., RT
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