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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 英文名:
Diethylmethylsilane
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
760-32-7
- 规格:
1g
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文献和实验Isolation of PBMCs from patient blood and buffy coats
3| Centrifuge at 760g for 20 min at room temperature (21 ± 5 1C). Slow acceleration and do not use brake. 4| Collect the cell band on top of the Ficoll layer with a Pasteur pipette and transfer it to a new 50-ml tube (maximum of 2 cell bands per tube
duplex PCR amplification, which is the coamplification of a fragment from the region of interest and a control fragment (e.g., the actin gene, or the tubulin gene). This approach allows for estimating relative levels of specific histone modifications
【精华】vol 687 Chapter 3 采用Splinkerette-PCR技术对前病毒基因组插入位点进行分离
., and vanLohuizen, M. (2002) Genome-wide retroviral insertional tagging of genes involved in cancer in Cdkn2a-deficient mice. Nat Genet 32, 160–5. 12. Ding, S., Wu, X., Li, G., Han, M., Zhuang,Y., and Xu, T. (2005) Efficient transpositionof the piggyBac (PB
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