N-Boc-L-亮氨醇;82010-31-9;97%;S50

二肽的概念与二肽合成的个人心得体会

-L-Tyrosine-OMe 将L-Tyrosine-OMe盐酸盐2.31g溶于15mlDMF，加入3ml的三乙胺，搅拌反应15min，加入3.05g的N-Boc-L-Tryptophan色氨酸和二氯甲烷的溶液，滴加5.67gN,N-二环己基亚酰胺(DCC)溶于10ml二氯甲烷的溶液，反应4小时,过滤。滤液减压浓缩,加入乙酸乙酯和水,水层以乙酸乙酯提取。将提取液与有机层合并，用10%柠檬酸、饱和食盐水、饱和碳酸氢钠依次洗涤。将液体干燥、浓缩,加入石油醚结晶。 当按着实验做

Hints and precautions for the care, feeding and breeding of Neurospora

and supplements. The synthetic crossing medium (SC) of Westergaard and Mitchell is most widely used. The need to adjust pH of SC to 6.5 can be avoided by substituting 0.7 g K2HP04 and 0.5 g KH2HP04 per liter for the monobasic salt in the original formula (1963 N.N

v468 Chapter 2 Wnt活性蛋白分离及应用

: L-Wnt3A (ATCC , Manassas, VA; ATCC# CRL-2647) (see Note 1 ). 2. Cell culture medium: Dulbecco’s minimum essential medium(DMEM) supplemented with 10% (v/v) fetal bovine serum(FBS), 1:100 dilution of penicillin–streptomycin solution with10,000 U