- ￥456.90
- 源叶
- 中国
- K30868-10μg
- 2025年10月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
2-8°C;避光;禁止冻结
- 英文名：
I-A/I-E Rat mAb (PE-Cy7)
- 库存：
99
- 供应商：
上海源叶生物科技有限公司
- 规格：
10μg
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
cold wash buffer (PBS/0.1% NaN3/1.0% fetal bovine serum). Centrifuge at 350 x g for 5 min. Finally, resuspend cell pellet to a concentration of 2 x 107 cells/ml (i.e., 106 cells per 50 µl). Dilute primary mAbs (e.g., unconjugated, biotinylated
Cell Surface Immunofluorescence Staining Protocol
. 2. Lyse Red Cells: 1) If necessary (e.g. spleen), dilute 10X Red Blood Cell (RBC) Lysis Buffer (BioLegend Cat. #420301) to 1X working concentration with DI water and resuspend pellet in 3 ml 1X RBC Lysis Buffer. Incubate on ice
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
anti-DNA-POD (1: 5; 1: 10) overnight at 4°C. 7. Wash in PBS (3x5 min). 8. Demonstration of peroxidase activity in a Petri dish: dissolve a DAB tablet (10 mg) in 40 ml of 0.1M citrate acid-sodium citrate buffer (pH 5.5), add 5 ml of H2
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