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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C;避光;禁止冻结
- 英文名:
I-A/I-E Rat mAb (PE-Cy5)
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μg
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文献和实验Dual- and Triple-Color Fluorospot
in the analysis, e.g., IL-10, rat mAb 9D7 is used for coating and tag (different from FITC and biotin)-labeled rat mAb 12G8 is used for detection. All mAbs from Mabtech, Nacka Strand, Sweden. 4. Secondary reagents for amplification: Mouse anti-FITC mAb
Immunofluorescent Staining of Mouse and Rat Leukocytes
cold wash buffer (PBS/0.1% NaN3/1.0% fetal bovine serum). Centrifuge at 350 x g for 5 min. Finally, resuspend cell pellet to a concentration of 2 x 107 cells/ml (i.e., 106 cells per 50 µl). Dilute primary mAbs (e.g., unconjugated, biotinylated
Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
anti-DNA-POD (1: 5; 1: 10) overnight at 4°C. 7. Wash in PBS (3x5 min). 8. Demonstration of peroxidase activity in a Petri dish: dissolve a DAB tablet (10 mg) in 40 ml of 0.1M citrate acid-sodium citrate buffer (pH 5.5), add 5 ml of H2
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