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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C;避光;禁止冻结
- 英文名:
Rat IgG (APC-Cy7)
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
10μg
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文献和实验Cell Surface Immunofluorescence Staining Protocol
for 5 minutes. 2) Stop cell lysis by adding 10 ml Cell Staining Buffer to the tube. Centrifuge for 5 minutes at 350 x g and discard supernatant. 3) Repeat wash as in step 2. 4) Count viable cells and resuspend in Cell
, 10 μg/mL leupeptin, 1 mM phenylmethanesulfonylfluoride (PMSF), and 1 mM sodium orthovanadate. 6. Teflon cell scrapers (Fisher, Pittsburgh, PA ). 7. 2× SDS sample buffer for electrophoresis: 0.0375 M Tris-HCl, pH 6.5, 8% (w/v) SDS, 10% (v/v
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
:chloroform:isoamyl alcohol, UltraPure (25:24:1 v/v/v; Invitrogen) Phosphate-buffered saline (PBS; GIBCO 20012) Protease inhibitor cocktail (Sigma P8340) Proteinase K (10 mg/mL in TE buffer, pH 8.0; Invitrogen) PureLink HQ 96 Mini
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