thrombin;0;K22469-500μg

GST Fusion Protein Preparation

plus thrombin.10μg per mg fusion protein is a good starting point.Incubate 30-60 minutes at room temp rocking on the Nutator.If the protein is subject to degradation,try a 60 minute cleavage at 4℃.Drain BioRad columns.Soluble cleaved fusion protein

Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sar

to obtain 200–500 μg/L of yeast culture of a 50% pure SERCA1a that displays an ATPase activity similar to that of the native rabbit Ca2+ -ATPase. To succeed in crystallization, an additional size exclusion chromatography step was necessary. This step

Clot Retraction Test

  Use either the whole blood or platelet-rich plasma (PRP) To obtain PRP, collect 500 µl of blood on 50 µl of ACD Add 600 µl of PBS, centrifuge once at 10ѓC, 1200 rpm ( Joan, GR 412) In Glass Centrifuge Tube add 100