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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 英文名:
thrombin
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- 规格:
500μg
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文献和实验GST Fusion Protein Preparation
plus thrombin.10μg per mg fusion protein is a good starting point.Incubate 30-60 minutes at room temp rocking on the Nutator.If the protein is subject to degradation,try a 60 minute cleavage at 4℃.Drain BioRad columns.Soluble cleaved fusion protein
to obtain 200–500 μg/L of yeast culture of a 50% pure SERCA1a that displays an ATPase activity similar to that of the native rabbit Ca2+ -ATPase. To succeed in crystallization, an additional size exclusion chromatography step was necessary. This step
Use either the whole blood or platelet-rich plasma (PRP) To obtain PRP, collect 500 µl of blood on 50 µl of ACD Add 600 µl of PBS, centrifuge once at 10ѓC, 1200 rpm ( Joan, GR 412) In Glass Centrifuge Tube add 100
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