NPC-567;109333-26-8;0;K21845-5

Lambda（噬菌体）DNA Miniprep

David Harry Institute of Forest Genetics USDA Forest Service Pacific Southwest Research Station August 26, 1993 Background : There are many published methods for mini-preps of DNA from lambda phage cloning vectors. This one has worked

v468 Chapter 2 Wnt活性蛋白分离及应用

. In an optimal experiment, the Wnt proteincan be enriched 2,000- to 2,500-fold (see Notes 4 and 5 ). 1. Column Packing: Pour a 1:1 slurry of the Blue Sepharose intoa clean and empty column, such as XK26-20 or XK50-20 (GEHealthcare). Allow resin to settle

Purification of Myosin Light Chain Kinase

)2 - 8ml of 1M stock solution 200 mM NaCl- 23.4g 1 mM DTT* - 308mg 10 μmleupeptin* - 2ml of 5mg/ml stock solution 0.1 mM PMSF* - 3.6ml of 10mg/ml stock solution 1 μg/ml Pepstatin A* - 2ml of 1mg/ml stock solution pH 7.0 9.PMSF 500 mg 10mg/ml 50 ml