Ziyuglycoside II;35286-59-0;10

人APC和TSC－2 cDNA的长5＇RACE

的同聚核苷酸锚定序列。利用第二个巢式GSP和一个与同聚核苷酸尾巴可以退火的锚定引物来扩增cDNA的5＇末端。用SUPERSCRIPTTM II RT从mRNA复制成cDNA用RNase H和RNase T1降解RNA用GLASSMAXò Spin Cartridge纯化cDNA用dCTP和TdT给纯化的cDNA加尾用精简的锚定引物和巢式GSP2 PCR扩增dC加尾的cDNA 用AUAP和巢式GSP重新扩增初步PCR产物最初的5＇RACE系统是按照这个方法进行的，它首先利用SUPERSCRIPT™

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

1a).Part II Triple immunoprecipitation: H3K27 ChIP/-flag-DNMT3A/biotin-RNA A total of 4.0×106 293T cells are plated out and 24 hours later transfected plated (~70% confluent at the time of transfection) with the flag-tagged protein expressing plasmid

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution