3,4-Dihydro-4-oxo-;5368-37-6;9

Native Chromatin Preparation and Illumina/Solexa Library Construction

Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer

Methods for DNA isolation

or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture

碱裂法提取BAC

off supernatant and drain or wipe out excess supernatant. Should see pellet at this point. Dissolve the pellet in 700 µl of 50T/50E (50 mM Tris/50 mM EDTA, pH 8). Add 10 µl of RNase (10mg/ml) and leave at 37°C in a water bath for 1 hour. 11. Transfer the DNA