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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT
- 英文名:
2-Bromo-2-cyclopentenone
- 库存:
99
- 供应商:
上海源叶生物科技有限公司
- CAS号:
10481-34-2
- 规格:
100mg
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文献和实验磷酸酶缓冲液:100 mmol/L Tris-base,100 mmol/L NaCl,5 mmol/L MgCl2( pH 9.8 ) 含有 0.33 mg/ml NBT (Nitro blue tetrazolium ) ,0.16 mg/ml BCIP (5 - bromo - 4 -chloro-3 -indolyl phosphate)。 ( 7 ) GUS底物:10 mmol/L EDTA ( pH 7.0 ) ,0.1 mol/L phosphate buffer (pH
Recombineering‐Based Procedure for Creating BAC Transgene Constructs for Animals and Cell Lines
) Agar plates (unit 1.1 ) 100 µg/ml ampicillin (50 mg/ml stock in water; Sigma) Phusion high‐fidelity
). This is probably the most-cited unrefereed abstract! It contains approximately 4% formaldehyde and 5% glutaraldehyde in approximately 0.1 M phosphate or cacodylate buffer. Final pH = 7.2. If cacodylate (toxic!) is used, add calcium chloride (0.5 mg/ml
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