- 询价
- STEMCELL Technologies
- 07183
- 加拿大
- 2025年12月20日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
现货
- 供应商：
柏莱源（天津）生物科技有限公司
- 英文名：
CellAdhere™ Dilution Buffer
- 规格：
询价
将 CellAdhere™ 稀释缓冲液与 Vitronectin XF™ 细胞培养基质（由 Nucleus Biologics 开发和生产）配合使用，可为人胚胎干细胞（ES）和诱导多能干细胞（iPS）的培养提供成分明确、无动物源成分的培养表面。
使用 Vitronectin XF™（可作为 Corning® Matrigel® 的替代品，支持人多能干细胞的生长和分化）时必需配备 CellAdhere™ 稀释缓冲液。
| 成分说明 | CellAdhere™ 稀释缓冲液为化学成分明确溶液 |
| 适用细胞类型 | PSC衍生内胚层细胞, 多能干细胞 |
| 适用物种 | 人 |
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文献和实验[1]Slamecka J, Salimova L, McClellan S, van Kelle M, Kehl D, Laurini J, Cinelli P, Owen L, Hoerstrup SP, Weber B. Non-integrating episomal plasmid-based reprogramming of human amniotic fluid stem cells into induced pluripotent stem cells in chemically defined conditions. Cell Cycle. 2016;15(2):234-49. doi: 10.1080/15384101.2015.1121332. PMID: 26654216; PMCID: PMC4825845.
[2]Robinson M, Chapani P, Styan T, Vaidyanathan R, Willerth SM. Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells. Stem Cell Rev Rep. 2016 Aug;12(4):476-83. doi: 10.1007/s12015-016-9655-7. PMID: 27138845.
[3]Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Chemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011 May;8(5):424-9. doi: 10.1038/nmeth.1593. Epub 2011 Apr 10. PMID: 21478862; PMCID: PMC3084903.
Microbiology - Serial Dilution
. +900 mL Buf. +900 mL Buf. +900 mL Buf. Yield: 1000 mL 10-1 1000 mL 10-2 1000 mL 10-3 1000 mL 10-4 4. Since 100 mL of the 10-1 dilution is used to make the 10-2 dilution, only 900 mL remains for use. 5. The buffer used for dilution
Sample Protopcol for Two-Step Carbodiimide Coupling of Protein to MagPlex™ Magnetic Carboxylated Microspheres Microspheres should be protected from prolonged exposure to light throughout this procedure. 1.Resuspend
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
& alternative recipe at end for lysis buffer and IP Dilution Buffer) 2. Add acid-washed glass beads, so there is a 1 to 2mm layer of liquid above the beads. 3. Vortex in the Tomy Mixer. Set it to level 10, mix 8 times for 1 minute each, and put on ice
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