- ￥1200
- 博尔森生物
- BES2013K-2-2PL
- 中国/上海
- 2025年11月26日
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- 详细信息
- 技术资料
- 品系：
人
- 细胞类型：
正常细胞
- 肿瘤类型：
否
- 供应商：
上海博尔森生物科技有限公司
- 库存：
大量
- 英文名：
CX3CL1 Knockout HepG2 Cell Ripa Lysate (Pool)
- 生长状态：
详见操作说明
- 年限：
12个月
- 运输方式：
4℃
- 器官来源：
人
- 是否是肿瘤细胞：
否
- 细胞形态：
人
- 免疫类型：
详见操作说明
- 物种来源：
人
- 相关疾病：
详见操作说明
- 组织来源：
详见操作说明
- 规格：
50μL, 2μg/μl
CX3CL1基因敲除HepG2细胞RIPA裂解液（Pool）
CX3CL1 Knockout HepG2 Cell Ripa Lysate (Pool)
Catalog Number BES2013K-2-2PLCX3CL1 Knockout HepG2 Cell Ripa Lysate (Pool)
Gene Symbol CX3CL1
Synonyms C3Xkine; Chemokine C X3 C motif ligand 1; Chemokine CX3C Motif Ligand 1; CX3C membrane anchored chemokine; CXC 3; CXC3; CXC3C; FKN.
Swiss P78423
Gene ID 6376
gRNA Species Human
Parental Cell line HepG2
Knockout Region E3
Knockout Validation PCR + Sanger Sequencing
Mutation Description Knockout achieved by using CRISPR/Cas9, Deletion of the selection cassette in exon 3.
Applications: WB
Packaging 1 vial knockout cell Lysate and 1 vial parental cell Lysate.
Shipping Conditions 4℃
Storage Store at -20°C for 6 months storage or -80°C for long-term storage.
Notes This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.
Protocol Lysates are made using Ripa buffer with 1mM Phenylmethylsulfonyl fluoride (PMSF).
Proteins in this buffer are denatured.To use as WB control, SDS-PAGE loading buffer should be added to the lysate and boiled for 5 minutes, then loaded with gel.
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CX3CL1 Knockout HepG2 Cell Ripa Lysate (Pool)
￥1200
